The purpose of this paper is the description of Anostirus bohemicus sp. n. from the area of northwestern Czechia and Anostirus platiai sp. n. from Italy. In addition, the occurrence and morphology of some species of the genus Anostirus C. G. Thomson, 1859, known from Alpine region, Apennines, French Massif Central and Pyrenees are discussed: Anostirus colacurcioi Platia & Pedroni, 2009, A. gabilloti (Pic, 1907), A. gudenzii Platia, 1983, A. lauianus Wurst, 1994, A. maccapanii Platia, 2017, A. marginatus Pic, 1931, A. pseudosulphuripennis Binaghi, 1940, A. sulphuripennis sulphuripennis (Germar, 1843), A. sulphuripennis penninus Binaghi, 1940, A. reissi (Reitter, 1913), A. zenii zenii (Rosenhauer, 1856) and A. zenii binaghianus Wurst, 1995. The text is supplemented with new, previously unpublished photographs of the above species. An overview of all records of A. sulphuripennis sulphuripennis from Czechia is given.

In this paper a review of European species of the genus Aplotarsus Stephens, 1830 is given: Aplotarsus angustulus (Kiesenwetter, 1858), A. incanus (Gyllenhal, 1827) and A. tibiellus (Chevrolat, 1865). Aplotarsus angustulus is a new species for Armenia. Aplotarsus incanus is a new species for Bulgaria. The morphological variability of Aplotarsus incanus is discussed. An overview of all records of Aplotarsus angustulus and A. incanus in the Czechia and Slovakia is given, the biotopes of this click-beetle species are described in detail and photo-documented.

The last comprehensive list of insect species recorded from these reserves was published by Háva (2021), and supplements to this publication were published later (2022, 2023). In the present paper, the author presents 43 newly identified or confirmed species belonging to the orders Coleoptera, Diptera and Lepidoptera.